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KMID : 0380020070220050366
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Korean Journal of Biotechnology and Bioengineering
2007 Volume.22 No. 5 p.366 ~ p.369
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Reuse of Eluent by Controlling its Specific Gravity during the Chromatographic Purification of Paclitaxel
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Kim Jin-Hyun
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Abstract
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In this study, the feasibility of reusing the eluent was confirmed by monitoring its specific gravity during the chromatographic purification of paclitaxel from plant cell cultures. The specific gravity of the eluent (methanol/water = 70/30, v/v) was measured prior to its elution through the hydrophobic resin column. The measurement showed a specific gravity of 0.853. The discharged eluent from the column outlet was first evaporated under vacuum pressure. The evaporated eluent was collected and condensed into a liquid eluent again, followed by the HPLC analysis in order to check the presence of any trace of impurity. Even if the specific gravity of the liquid eluent is varied from 0.853 as a result of the evaporation and condensation, the eluent can still be reused after it specific gravity is adjusted by the addition of methanol or water. The reuse of the eluent resulted in the paclitaxel yield of 86% with a purity of 95% which were closely similar to those of before the eluent reuse. These results indicate that the strategy of reusing the eluent on the basis of the specific gravity analysis was successfully implemented in this study.
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KEYWORD
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Paclitaxel, chromatographic purification, eluent reuse, specific gravity
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